trifolii . enzymes in Rhizobium leguminosarum biovar Cell - associated pectinolytic and cellulolytic
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Cell-bound Cellulase and Polygalacturonase Production by Rhizobium and Brad Yrhizobium Species
Carboxymethyl cellulase and polygalacturonase activities were evaluated in wild-type strains from different taxonomic groups of rhizobia which nodulate a specific range of legume hosts that do not normally overlap (R. meliloti, R. loti, R. leguminosarum biovar trifolii, R. leguminosarum biovar phaseoli, R. leguminosarum biovar viceae, and Bradyrhizobium japonicum). To detect these enzymatic act...
متن کاملCell-associated pectinolytic and cellulolytic enzymes in Rhizobium leguminosarum biovar trifolii.
The involvement of Rhizobium enzymes that degrade plant cell wall polymers has long been an unresolved question about the infection process in root nodule symbiosis. Here we report the production of enzymes from Rhizobium leguminosarum bv. trifolii that degrade carboxymethyl cellulose and polypectate model substrates with sensitive methods that reliably detect the enzyme activities: a double-la...
متن کاملDirect amplification of nodD from community DNA reveals the genetic diversity of Rhizobium leguminosarum in soil.
Sequences of nodD, a gene found only in rhizobia, were amplified from total community DNA isolated from a pasture soil. The polymerase chain reaction (PCR) primers used, Y5 and Y6, match nodD from Rhizobium leguminosarum biovar trifolii, R. leguminosarum biovar viciae and Sinorhizobium meliloti. The PCR product was cloned and yielded 68 clones that were identified by restriction pattern as deri...
متن کاملUse of the Chrome Azurol S Agar Plate Technique To Differentiate Strains and Field Isolates of Rhizobium leguminosarum biovar trifolii.
Identification of Rhizobium and Bradyrhizobium strains and especially of indigenous isolates continues to be one of the major difficulties associated with competition studies. Because there is no universally accepted method, the method of choice depends on preference, experience, and equipment. Here, an agar plate technique was used to distinguish strains and field isolates of Rhizobium legumin...
متن کاملThe Rhizobium Ieguminosarum biovar viciae nod0 gene can enable a nod€ mutant of Rhizobium leguminosarum biovar trifolii to nodulate vetch
Sciences, University of East Anglia, Norwich NR4 7TJ, UK 2 School of Biological Analysis of the nodulation characteristics of transposon-induced mutants of Rhizobium leguminosarum bv. viciae revealed that nodO and the closely-linked rhi genes contribute t o nodulation of peas (Pisum sativum) and the vetch Vicia hirsuta. Although mutation of nodO alone had no significant effect on nodulation of ...
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